Anne Skaja Robinson

TitleProfessor
InstitutionUniversity of Delaware
DepartmentChemical and Biomolecular Engineering
Address150 Academy St.
Newark DE 19716
Phone302-831-0557
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    Other Positions
    TitleProfessor
    InstitutionUniversity of Delaware
    DepartmentDelaware Biotechnology Institute (DBI)

    TitleAssociate Chair for Biochemical Engineering
    InstitutionUniversity of Delaware
    DepartmentCollege of Engineering


    Collapse Research 
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    F32GM017538     (ROBINSON, ANNE SKAJA)Mar 18, 1996
    NIH
    NOVEL ROLE FOR CYSTEINE IN PROTEIN FOLDING ASSEMBLY
    Role: Principal Investigator

    R01GM060543     (ROBINSON, ANNE SKAJA)Sep 30, 2000 - Aug 31, 2004
    NIH
    NOVEL CYSTEINES OF P22 TAILSPIKE PROTEIN
    Role: Principal Investigator

    R01GM065507     (ROBINSON, ANNE SKAJA)Mar 1, 2002 - Feb 28, 2006
    NIH
    Sensing and Analyzing Stress During Protein Expression
    Role: Principal Investigator

    R01GM075297     (ROBINSON, ANNE SKAJA)Apr 1, 2005 - Mar 31, 2010
    NIH
    Stochastic Modeling of Protein Interactions in the Endoplasmic Reticulum
    Role: Principal Investigator

    F33AG031610     (ROBINSON, ANNE SKAJA)Oct 1, 2007 - Sep 30, 2008
    NIH
    Tau Homeostasis and Neurodegeneration
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. O'Brien CJ, Rubio CC, Razinkov VI, Robinson AS, Roberts CJ. Biophysical characterization and molecular simulation of electrostatically driven self-association of a single-chain antibody. Protein Sci. 2018 Apr 10. PMID: 29637646.
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    2. Radhakrishnan D, Robinson AS, Ogunnaike BA. Controlling the Glycosylation Profile in mAbs Using Time-Dependent Media Supplementation. Antibodies (Basel). 2017 Dec 21; 7(1). PMID: 31544854.
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    3. Meric G, Robinson AS, Roberts CJ. Driving Forces for Nonnative Protein Aggregation and Approaches to Predict Aggregation-Prone Regions. Annu Rev Chem Biomol Eng. 2017 Jun 07; 8:139-159. PMID: 28592179.
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    4. McNeely PM, Naranjo AN, Forsten-Williams K, Robinson AS. A2AR Binding Kinetics in the Ligand Depletion Regime. SLAS Discov. 2017 Feb; 22(2):166-175. PMID: 27577981.
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    5. St Amand MM, Hayes J, Radhakrishnan D, Fernandez J, Meyer B, Robinson AS, Ogunnaike BA. Identifying a robust design space for glycosylation during monoclonal antibody production. Biotechnol Prog. 2016 Sep; 32(5):1149-1162. PMID: 27273898.
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    6. Naranjo AN, McNeely PM, Katsaras J, Robinson AS. Impact of purification conditions and history on A2A adenosine receptor activity: The role of CHAPS and lipids. Protein Expr Purif. 2016 Aug; 124:62-7. PMID: 27241126.
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    7. Wu H, Truncali K, Ritchie J, Kroe-Barrett R, Singh S, Robinson AS, Roberts CJ. Weak protein interactions and pH- and temperature-dependent aggregation of human Fc1. MAbs. 2015 Nov 2; 7(6):1072-83. PMID: 26267255.
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    8. Naranjo AN, Chevalier A, Cousins GD, Ayettey E, McCusker EC, Wenk C, Robinson AS. Conserved disulfide bond is not essential for the adenosine A2A receptor: Extracellular cysteines influence receptor distribution within the cell and ligand-binding recognition. Biochim Biophys Acta. 2015 Feb; 1848(2):603-14. PMID: 25445670.
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    9. St Amand MM, Radhakrishnan D, Robinson AS, Ogunnaike BA. Identification of manipulated variables for a glycosylation control strategy. Biotechnol Bioeng. 2014 Oct; 111(10):1957-70. PMID: 24728980.
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    10. Wu H, Kroe-Barrett R, Singh S, Robinson AS, Roberts CJ. Competing aggregation pathways for monoclonal antibodies. FEBS Lett. 2014 Mar 18; 588(6):936-41. PMID: 24530501.
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    11. St Amand MM, Tran K, Radhakrishnan D, Robinson AS, Ogunnaike BA. Controllability analysis of protein glycosylation in cho cells. PLoS One. 2014; 9(2):e87973. PMID: 24498415.
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    12. Blanco MA, Sahin E, Robinson AS, Roberts CJ. Coarse-grained model for colloidal protein interactions, b22, and protein cluster formation. J Phys Chem B. 2013 Dec 19; 117(50):16013-28. PMID: 24289039.
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    13. Maurer RW, Hunter AK, Robinson AS, Roberts CJ. Aggregates of a-chymotrypsinogen anneal to access more stable states. Biotechnol Bioeng. 2014 Apr; 111(4):782-91. PMID: 24122552.
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    14. Morozova OA, March ZM, Robinson AS, Colby DW. Conformational features of tau fibrils from Alzheimer's disease brain are faithfully propagated by unmodified recombinant protein. Biochemistry. 2013 Oct 8; 52(40):6960-7. PMID: 24033133.
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    15. Young CL, Raden DL, Robinson AS. Analysis of ER Resident Proteins in Saccharomyces cerevisiae: Implementation of H/KDEL Retrieval Sequences. Traffic. 2013 Apr; 14(4):365-81. PMID: 23324027.
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    16. McNeely PM, Naranjo AN, Robinson AS. Structure-function studies with G protein-coupled receptors as a paradigm for improving drug discovery and development of therapeutics. Biotechnol J. 2012 Dec; 7(12):1451-61. PMID: 23213015.
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    17. Britton ZT, Hanle EI, Robinson AS. An expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization. Protein Expr Purif. 2012 Aug; 84(2):224-35. PMID: 22722102.
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    18. Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS. Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Yeast. 2012 Mar; 29(3-4):119-36. PMID: 22473760.
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    19. Young CL, Britton ZT, Robinson AS. Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications. Biotechnol J. 2012 May; 7(5):620-34. PMID: 22442034.
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    20. O'Malley MA, Helgeson ME, Wagner NJ, Robinson AS. Toward rational design of protein detergent complexes: determinants of mixed micelles that are critical for the in vitro stabilization of a G-protein coupled receptor. Biophys J. 2011 Oct 19; 101(8):1938-48. PMID: 22004748.
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    21. Robinson A. New tools for breaking barriers to GPCR expression in E. coli. J Mol Biol. 2011 May 13; 408(4):597-8. PMID: 21420418.
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    22. Sahin E, Jordan JL, Spatara ML, Naranjo A, Costanzo JA, Weiss WF, Robinson AS, Fernandez EJ, Roberts CJ. Computational design and biophysical characterization of aggregation-resistant point mutations for ?D crystallin illustrate a balance of conformational stability and intrinsic aggregation propensity. Biochemistry. 2011 Feb 8; 50(5):628-39. PMID: 21184609.
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    23. Young CL, Yuraszeck T, Robinson AS. Decreased secretion and unfolded protein response upregulation. Methods Enzymol. 2011; 491:235-60. PMID: 21329804.
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    24. O'Malley MA, Naranjo AN, Lazarova T, Robinson AS. Analysis of adenosine A2a receptor stability: effects of ligands and disulfide bonds. Biochemistry. 2010 Nov 2; 49(43):9181-9. PMID: 20853839.
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    25. O'Malley MA, Mancini JD, Young CL, McCusker EC, Raden D, Robinson AS. Progress toward heterologous expression of active G-protein-coupled receptors in Saccharomyces cerevisiae: Linking cellular stress response with translocation and trafficking. Protein Sci. 2009 Nov; 18(11):2356-70. PMID: 19760666.
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    26. Xu P, Robinson AS. Decreased secretion and unfolded protein response up-regulation are correlated with intracellular retention for single-chain antibody variants produced in yeast. Biotechnol Bioeng. 2009 Sep 1; 104(1):20-9. PMID: 19415776.
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    27. McCusker E, Robinson AS. Refolding of G protein alpha subunits from inclusion bodies expressed in Escherichia coli. Protein Expr Purif. 2008 Apr; 58(2):342-55. PMID: 18249008.
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    28. O'Malley MA, Lazarova T, Britton ZT, Robinson AS. High-level expression in Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional human adenosine A2a receptor. J Struct Biol. 2007 Aug; 159(2):166-78. PMID: 17591446.
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    29. McCusker EC, Bane SE, O'Malley MA, Robinson AS. Heterologous GPCR expression: a bottleneck to obtaining crystal structures. Biotechnol Prog. 2007 May-Jun; 23(3):540-7. PMID: 17397185.
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    30. Powers SL, Robinson AS. PDI improves secretion of redox-inactive beta-glucosidase. Biotechnol Prog. 2007 Mar-Apr; 23(2):364-9. PMID: 17315887.
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    31. Bane SE, Velasquez JE, Robinson AS. Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli. Protein Expr Purif. 2007 Apr; 52(2):348-55. PMID: 17166740.
      Citations:    
    32. Powers SL, Robinson CR, Robinson AS. Denaturation of an extremely stable hyperthermophilic protein occurs via a dimeric intermediate. Extremophiles. 2007 Jan; 11(1):179-89. PMID: 17072686.
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    33. Wedekind A, O'Malley MA, Niebauer RT, Robinson AS. Optimization of the human adenosine A2a receptor yields in Saccharomyces cerevisiae. Biotechnol Prog. 2006 Sep-Oct; 22(5):1249-55. PMID: 17022661.
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    34. Kim J, Robinson AS. Dissociation of intermolecular disulfide bonds in P22 tailspike protein intermediates in the presence of SDS. Protein Sci. 2006 Jul; 15(7):1791-3. PMID: 16751612.
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    35. Niebauer RT, Robinson AS. Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae. Protein Expr Purif. 2006 Apr; 46(2):204-11. PMID: 16289981.
      Citations:    
    36. Smith JD, Richardson NE, Robinson AS. Elevated expression temperature in a mesophilic host results in increased secretion of a hyperthermophilic enzyme and decreased cell stress. Biochim Biophys Acta. 2005 Aug 31; 1752(1):18-25. PMID: 16112628.
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    37. Gage MJ, Zak JL, Robinson AS. Three amino acids that are critical to formation and stability of the P22 tailspike trimer. Protein Sci. 2005 Sep; 14(9):2333-43. PMID: 16081648.
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    38. Xu P, Raden D, Doyle FJ, Robinson AS. Analysis of unfolded protein response during single-chain antibody expression in Saccaromyces cerevisiae reveals different roles for BiP and PDI in folding. Metab Eng. 2005 Jul; 7(4):269-79. PMID: 15990348.
      Citations:    
    39. Niebauer RT, Wedekind A, Robinson AS. Decreases in yeast expression yields of the human adenosine A2a receptor are a result of translational or post-translational events. Protein Expr Purif. 2004 Sep; 37(1):134-43. PMID: 15294291.
      Citations:    
    40. Lefebvre BG, Comolli NK, Gage MJ, Robinson AS. Pressure dissociation studies provide insight into oligomerization competence of temperature-sensitive folding mutants of P22 tailspike. Protein Sci. 2004 Jun; 13(6):1538-46. PMID: 15133163.
      Citations:    
    41. Lefebvre BG, Gage MJ, Robinson AS. Maximizing recovery of native protein from aggregates by optimizing pressure treatment. Biotechnol Prog. 2004 Mar-Apr; 20(2):623-9. PMID: 15059011.
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    42. Smith JD, Tang BC, Robinson AS. Protein disulfide isomerase, but not binding protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast. Biotechnol Bioeng. 2004 Feb 5; 85(3):340-50. PMID: 14748090.
      Citations:    
    43. Gage MJ, Robinson AS. C-terminal hydrophobic interactions play a critical role in oligomeric assembly of the P22 tailspike trimer. Protein Sci. 2003 Dec; 12(12):2732-47. PMID: 14627734.
      Citations:    
    44. Butz JA, Niebauer RT, Robinson AS. Co-expression of molecular chaperones does not improve the heterologous expression of mammalian G-protein coupled receptor expression in yeast. Biotechnol Bioeng. 2003 Nov 5; 84(3):292-304. PMID: 12968283.
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    45. Danek BL, Robinson AS. Nonnative interactions between cysteines direct productive assembly of P22 tailspike protein. Biophys J. 2003 Nov; 85(5):3237-47. PMID: 14581223.
      Citations:    
    46. Lefebvre BG, Robinson AS. Pressure treatment of tailspike aggregates rapidly produces on-pathway folding intermediates. Biotechnol Bioeng. 2003 Jun 5; 82(5):595-604. PMID: 12652483.
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    47. Sinacola JR, Robinson AS. Rapid refolding and polishing of single-chain antibodies from Escherichia coli inclusion bodies. Protein Expr Purif. 2002 Nov; 26(2):301-8. PMID: 12406685.
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    48. Smith JD, Robinson AS. Overexpression of an archaeal protein in yeast: secretion bottleneck at the ER. Biotechnol Bioeng. 2002 Sep 30; 79(7):713-23. PMID: 12209794.
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    49. Kauffman KJ, Pridgen EM, Doyle FJ, Dhurjati PS, Robinson AS. Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae. Biotechnol Prog. 2002 Sep-Oct; 18(5):942-50. PMID: 12363344.
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