Two-Hybrid System Techniques

"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity.

MeSH information

Definition | Details | More General Concepts | Related Concepts | More Specific Concepts

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

Descriptor ID	D020798
MeSH Number(s)	E05.393.220.870 E05.601.690.650 E05.601.870
Concept/Terms	Two-Hybrid System Techniques Two-Hybrid System Techniques Technique, Two-Hybrid System Techniques, Two-Hybrid System Two Hybrid System Techniques Two-Hybrid System Technique Two-Hybrid Method Method, Two-Hybrid Methods, Two-Hybrid Two Hybrid Method Two-Hybrid Methods Two-Hybrid System Technics Technic, Two-Hybrid System Technics, Two-Hybrid System Two Hybrid System Technics Two-Hybrid System Technic Two-Hybrid Assay Assay, Two-Hybrid Assays, Two-Hybrid Two Hybrid Assay Two-Hybrid Assays One-Hybrid System Techniques One-Hybrid System Techniques One Hybrid System Techniques One-Hybrid System Technique Technique, One-Hybrid System Techniques, One-Hybrid System One-Hybrid System Technics One Hybrid System Technics One-Hybrid System Technic Technic, One-Hybrid System Technics, One-Hybrid System Reverse One-Hybrid System Techniques Reverse One-Hybrid System Techniques Reverse One Hybrid System Techniques Yeast Two-Hybrid System Techniques Yeast Two-Hybrid System Techniques Yeast Two Hybrid System Techniques Yeast Two-Hybrid System System, Yeast Two-Hybrid Systems, Yeast Two-Hybrid Two-Hybrid System, Yeast Two-Hybrid Systems, Yeast Yeast Two Hybrid System Yeast Two-Hybrid Systems Yeast Two-Hybrid Assay Assay, Yeast Two-Hybrid Assays, Yeast Two-Hybrid Two-Hybrid Assay, Yeast Two-Hybrid Assays, Yeast Yeast Two Hybrid Assay Yeast Two-Hybrid Assays Reverse Two-Hybrid System Techniques Reverse Two-Hybrid System Techniques Reverse Two Hybrid System Techniques Three-Hybrid System Techniques Three-Hybrid System Techniques Technique, Three-Hybrid System Techniques, Three-Hybrid System Three Hybrid System Techniques Three-Hybrid System Technique Tri-Hybrid System Techniques Technique, Tri-Hybrid System Techniques, Tri-Hybrid System Tri Hybrid System Techniques Tri-Hybrid System Technique Three-Hybrid System Technics Technic, Three-Hybrid System Technics, Three-Hybrid System Three Hybrid System Technics Three-Hybrid System Technic Yeast One-Hybrid System Techniques Yeast One-Hybrid System Techniques Yeast One Hybrid System Techniques Yeast Three-Hybrid System Techniques Yeast Three-Hybrid System Techniques Yeast Three Hybrid System Techniques Yeast Three-Hybrid System System, Yeast Three-Hybrid Systems, Yeast Three-Hybrid Three-Hybrid System, Yeast Three-Hybrid Systems, Yeast Yeast Three Hybrid System Yeast Three-Hybrid Systems Yeast Three-Hybrid Assay Assay, Yeast Three-Hybrid Assays, Yeast Three-Hybrid Three-Hybrid Assay, Yeast Three-Hybrid Assays, Yeast Yeast Three Hybrid Assay Yeast Three-Hybrid Assays n-Hybrid System Techniques n-Hybrid System Techniques System Technique, n-Hybrid System Techniques, n-Hybrid Technique, n-Hybrid System Techniques, n-Hybrid System n Hybrid System Techniques n-Hybrid System Technique Reverse Three-Hybrid System Techniques Reverse Three-Hybrid System Techniques Reverse Three Hybrid System Techniques

Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".

Analytical, Diagnostic and Therapeutic Techniques and Equipment [E]
Investigative Techniques [E05]
Genetic Techniques [E05.393]
Cloning, Molecular [E05.393.220]
Two-Hybrid System Techniques [E05.393.220.870]
Molecular Probe Techniques [E05.601]
Protein Interaction Mapping [E05.601.690]
Two-Hybrid System Techniques [E05.601.690.650]
Two-Hybrid System Techniques [E05.601.870]

Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".

publications

Timeline | Most Recent

This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications.

Bar chart showing 20 publications over 11 distinct years, with a maximum of 4 publications in 2002

To see the data from this visualization as text, click here.

Year	Major Topic	Minor Topic	Total
2002	0	4	4
2003	0	3	3
2004	0	2	2
2005	0	1	1
2006	0	2	2
2007	1	1	2
2008	0	2	2
2009	0	1	1
2010	0	1	1
2015	0	1	1
2017	0	1	1

Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.

Manthey GM, Clear AD, Liddell LC, Negritto MC, Bailis AM. Homologous recombination in budding yeast expressing the human RAD52 gene reveals a Rad51-independent mechanism of conservative double-strand break repair. Nucleic Acids Res. 2017 02 28; 45(4):1879-1888.

View in: PubMed
Thomas LR, Wang Q, Grieb BC, Phan J, Foshage AM, Sun Q, Olejniczak ET, Clark T, Dey S, Lorey S, Alicie B, Howard GC, Cawthon B, Ess KC, Eischen CM, Zhao Z, Fesik SW, Tansey WP. Interaction with WDR5 promotes target gene recognition and tumorigenesis by MYC. Mol Cell. 2015 May 7; 58(3):440-52.

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Orr CR, Montie HL, Liu Y, Bolzoni E, Jenkins SC, Wilson EM, Joseph JD, McDonnell DP, Merry DE. An interdomain interaction of the androgen receptor is required for its aggregation and toxicity in spinal and bulbar muscular atrophy. J Biol Chem. 2010 Nov 12; 285(46):35567-77.

View in: PubMed
Balakrishnan MP, Cilenti L, Mashak Z, Popat P, Alnemri ES, Zervos AS. THAP5 is a human cardiac-specific inhibitor of cell cycle that is cleaved by the proapoptotic Omi/HtrA2 protease during cell death. Am J Physiol Heart Circ Physiol. 2009 Aug; 297(2):H643-53.

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Evans BJ, Wang Z, Mobley L, Khosravi D, Fujii N, Navenot JM, Peiper SC. Physical association of GPR54 C-terminal with protein phosphatase 2A. Biochem Biophys Res Commun. 2008 Dec 26; 377(4):1067-71.

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Caplan JL, Mamillapalli P, Burch-Smith TM, Czymmek K, Dinesh-Kumar SP. Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector. Cell. 2008 Feb 8; 132(3):449-62.

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Ivanov AV, Peng H, Yurchenko V, Yap KL, Negorev DG, Schultz DC, Psulkowski E, Fredericks WJ, White DE, Maul GG, Sadofsky MJ, Zhou MM, Rauscher FJ. PHD domain-mediated E3 ligase activity directs intramolecular sumoylation of an adjacent bromodomain required for gene silencing. Mol Cell. 2007 Dec 14; 28(5):823-37.

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Eto M, Brautigan DL. Assay for three-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2. Methods Mol Biol. 2007; 365:197-208.

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Wu K, Liu M, Li A, Donninger H, Rao M, Jiao X, Lisanti MP, Cvekl A, Birrer M, Pestell RG. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol Biol Cell. 2007 Mar; 18(3):755-67.

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Fujimoto N, Terlizzi J, Aho S, Brittingham R, Fertala A, Oyama N, McGrath JA, Uitto J. Extracellular matrix protein 1 inhibits the activity of matrix metalloproteinase 9 through high-affinity protein/protein interactions. Exp Dermatol. 2006 Apr; 15(4):300-7.

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